Every step in the experiment is important, ignoring any one of them may lead to the failure of the experiment. Good habits can improve work efficiency and reduce experimental costs! Good experimental habits are also the foundation of successful experiments.
Sometimes many people know that their actions are wrong, but they have become accustomed to them or keep making mistakes to save trouble. This not only brings uncertainty to their experimental data, but also affects others, which is not worth the loss. Let's take a look at some bad habits in the laboratory? Have you been hit?
When it comes to the most common bad habits of experimenters, what comes to mind?
Let's take a look at the bad habits summarized by netizens together.
Have you been shot by these bad habits
Bad habits during the experimental process:
When weighing or measuring samples, record the data on a draft paper first, and after the sample is completed, copy it into a notebook;
Sometimes the experiment is completed before filling in the records uniformly;
Before the instrument drops below its limit, it begins to shut down;
When performing gas chromatography analysis, simply rub the needle of the syringe with your finger without wiping it with filter paper.
Bad habits in the pre-treatment process before the experiment:
When weighing samples, repeatedly use weighing paper;
Do not perform acid-base configuration in a fume hood;
Sometimes weighing or other operations are carried out without wearing gloves;
At the end of the volume adjustment process, a dropper is not used, but a washing bottle is directly used. The volume adjustment is not very accurate, and it often exceeds the scale line after being left to stand;
Before weighing, do not calibrate or check if the horizontal bubble is in the center zone.
Use a medicine spoon to retrieve the ingredients, and return any excess medicine to the bottle.
Bad habits after completing the experiment:
The laboratory waste liquid is directly poured into the sewage tank instead of being treated and recycled;
Disregarding the cleanliness of the table after the experiment is completed;
After using the balance, do not close the balance door.
Bad habits that affect others
The most important issue is the standardization of experimental operations, such as the conservation of reagents, the care of instruments, and so on. Mild cases involve problems with one's own experiments, while severe cases can affect the atmosphere of the laboratory. For example, if the experiment is not cleaned up in a timely manner after completion, it will always leave others with a mess; Long term occupation of laboratory public computers, online chatting and gaming, hindering others from searching for information; Public instruments are not taken care of, and after being damaged, they are concealed and not reported, shirking responsibility.
The bottle is not labeled, so I don't know what's inside.
The configuration information on the standard solution bottle is lazy and not updated.
Frequent and unplanned opening of the refrigerator, with the refrigerator door wide open for certain operations. Causing unstable temperature in the refrigerator, which affects the freezing effect of other stored items. Randomly rummaging through the items placed inside makes it difficult for later people to find their belongings.
The opening of the cell culture chamber door is too large, too frequent, and not closed in time, resulting in a decrease in the temperature and carbon dioxide concentration of the chamber. Observing cells cultivated by others without their consent. Sometimes, when cells are in need of stillness, unauthorized inspection by others may affect cell adhesion.
Items disinfected by others were opened without permission in the ultra clean bench. Not informing later and repackaging.
Gloves that are worn on hands and have toxic substances (such as EB, PMSF, etc.) stuck on them, including door handles, keyboards, etc. Causing others to indirectly adhere to such toxic substances with bare hands.
Bottles with toxic substances are not given special treatment and are placed together with other bottles.
Randomly writing on the label of public reagents, indicating one's own dosage, affects the use of future generations.
After using the centrifuge, microscope, etc., leave without turning them off.
Good habits that an experimenter should have
Before the experiment, it is important to read more materials and read several articles regularly. Do not wait until the end when the article is about to be published before reading; Before the experiment, fully consider the possible problems and results, design thoroughly, and carefully design the experiment. Do not forget to set up a control group!
During the experiment, observe carefully and not be influenced by one's own expected conclusions;
It's better to always have a pen in your pocket when conducting experiments. Yes, always keep a pen by your side and remember what needs to be memorized. Don't trust your own brain. If you choose to engage in scientific research, then you should be more careful. Science comes from practice, and practice is gradually integrated;
After the experiment is over, regardless of whether the results are good or bad, organize the data as soon as possible, process the data in a timely manner, analyze what needs to be statistically analyzed, and plot what needs to be plotted; Immediately or as soon as possible, organize the relevant images and put them together for easy viewing. It is important to organize electrophoresis and other patterns clearly to avoid forgetting what they are when using them.
I think when conducting experiments, it is important to understand the principles behind the experiments. By carefully recording each step of the experiment, one can identify the reasons for the experiment's failure. Every time, we demand results - either successful experiences or reasons for failure! Every success and mistake should be recorded in a notebook. Attitude is particularly important in experiments. If you want to succeed casually, the probability is almost zero. As the saying goes, attitude determines everything!
Summarize the situation every night and plan the experiment for the next day. Tomorrow's matter, decided today.
Refer to literature and be able to accurately explain one's own experimental results; If there are problems with the experiment, it cannot be blindly repeated. It should be carefully summarized and improved bit by bit;
I remember a NOBEL winner once said: When working in the laboratory, don't think about anything, focus on the task at hand; After conducting the experiment, one should have broad and imaginative associations, especially with new ideas and methods for the experiment.
Comply with laboratory protocols, promptly return items to their original positions, dispose of garbage and organize objects in a timely manner, and so on. Don't only focus on your own convenience, but also take care of others. Especially public items, they must be put back in place.
Do not easily use other people's things, greet them before use, keep detailed records, and record the batch number of reagents when preparing solutions; Try not to use other people's reagents as much as possible. Prepare all the reagents yourself, and it's easier to find the cause when there are problems. It is better to prepare reagents by oneself. When using common reagents once, quality inspection should be carried out, and the time and who prepared them should be indicated. Everyone in the laboratory should have a single letter abbreviation name.
Everything should be marked with the date, drug name, concentration, etc. in a timely manner.
It is better to only change one experimental condition at a time, otherwise it will be unclear which condition changed and affected the experimental results. Consciously fix the experimental conditions and try to replicate the experiment as much as possible.
Before conducting experiments with toxic drugs, please warn your classmates in the laboratory who are close to you, especially those without fume hoods, to leave or take protective measures;
Wash hands promptly after completing the experiment, clean the experimental site, wash and return the utensils to their original state, and turn off the power of instruments and other equipment.
Experience from predecessors
After finishing the experiment, dry the table and put everything back in place. Some things that can be recycled are recycled, and many laboratory gun heads are recycled.
At the end of each experiment, make timely records and do not wait. A good memory is not as good as a bad pen. Although this is a common saying, don't give up the importance of the pen!
After completing the experiment, clean the experimental platform so that you can continue working faster, better, and more conveniently next time! Put the medicine back in place, put the instrument back in place! Restore the used instruments to their original state.
After using the pipette, it should be returned to the large measuring position to prevent the spring from losing elasticity over time.
Pay attention to the safety of water and electricity before leaving or entering the laboratory!
Remember to turn off the water bath, remember to remember.
For the writing of published articles, when summarizing the content, each method and experimental result should be listed with its source, that is, where it comes from the experimental records. This is convenient for oneself, future reviewers, or future readers to have a trace when they have questions or communicate.
Internal information exchange in the laboratory: problems encountered during the experiment, recent literature information, and mutual communication and discussion.
The laboratory should have a summary of experimental methods for daily laboratory experiments and preparation of common supplies. In general, people use these methods, which are prone to errors. Once a problem arises, it is also easy to locate the problem. The result of using all kinds of methods in a mess is not conducive to internal communication in the laboratory.
Conducting experiments in the field of biology requires both self-protection and avoiding contamination of the laboratory environment to protect others.